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Requirements for TFII-I stimulation of −98 ERSE. COS cells were transfected either with (−109/−74)MCAT containing the wild-type (wt) rat grp78 −98 ERSE as the reporter gene (left panel) or with the GGC mutant [GGC(m)] of −98 ERSE as the reporter gene (right panel). The cells were transfected with either the empty vector, the wild-type GST–TFII-I, or the tyrosine phosphorylation site mutant (mt) of GST-TFII-I (YY-FF) as indicated. Error bars indicate standard deviations.
