FIG. 8.

TFII-I is an ERSE binding protein. (A) Antibody inhibition of ERSF complex formation. EMSAs were performed using HeLa NE from Tg-treated cells and ³²P-labeled −98 ERSE as a probe. Lane 1, standard EMSA reaction; lane 2, preincubation of the extract with 2 μl of normal rabbit serum (NRS); lane 3, binding reaction with 2 μl of the anti-TFII-I antibody. The positions of the ERSF, NF-Y, and YY1 complexes are indicated. (B) Binding of GST–TFII-I to −98 ERSE. An EMSA is presented for reactions of ³²P-labeled −98 ERSE as a probe and GST–TFII-I purified from extracts of COS cells transfected with either the empty vector (lane 2) or the expression vector for GST-TFII-I (lane 3). The band detected in lane 3 comigrated with the ERSF complex formed between −98 ERSE and HeLa NE prepared from Tg-treated cells (lane 1).