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. 2021 Dec 9;9:783385. doi: 10.3389/fcell.2021.783385

FIGURE 5.

FIGURE 5

In HCC, DUXAP8 acts as a molecular sponge for miR-584-5p, which downregulates miR-584-5p and affects the malignant phenotype and chemosensitivity of HCC. (A) The heat map demonstrating the differentially expressed microRNAs in HCC after DUXAP8 downregulation, and Wayne plot showing the differentially expressed microRNAs after the intersection of RNA sequencing results with the bioinformatics database. (B) Correlation analysis between the expression of DUXAP8 and miR-584-5p. (C) Dual-luciferase reporter assay detecting the relative activity of luciferase in transfected HCC cells. (D) Effect of DUXAP8 downregulation and overexpression on miR-584-5p expression in HCC cells. (E) RIP assay detecting the relative enrichment of DUXAP8 and miR-584-5p in anti-IgG or anti-AGO2 specific immunoprecipitates. (F) RNA pull-down assay was used to detect the interrelationship between DUXAP8, miR-584-5p, and AGO2. (G,H) Effects of DUXAP8 and miR-584-5p downregulation on the migration, invasion, primary and secondary spheroid-forming ability, and stemness-related gene expression levels in Huh7 cells. (I) The viability of HCC cells with downregulated DUXAP8 and miR-584-5p under the effect of sorafenib. Data are expressed as mean, *p < 0.05, **p < 0.01.