TABLE 4.
Important non-coding microRNAs reported in pig spermatozoa by transcriptome analysis.
| Species | Study type | Groups | MicroRNAs | Function | References |
|---|---|---|---|---|---|
| Pigs | qRT-PCR | Ejaculated spermatozoa | let-7a, -7d, and -7e | Spermatogenesis | Curry et al. (2011) |
| miR-22 | Sperm structure | ||||
| Epididymal and ejaculated sperm | let-7a and miR-92a | Calcium and camp signalling | Chang et al. (2016) | ||
| ssc-let-7a, ssc-let-7d, ssc-let-7e and ssc-miR-98 regulate | Sperm apoptosis | ||||
| miR-19 and miR-26a | AKT/PKB signalling pathway | ||||
| miR-224, miR-19b,miR-504 and miR-676 | Cell apoptosis and cell proliferation | ||||
| RNA-Seq | Fresh vs frozen spermatozoa | miR-26a, and miR-455-5p | PTEN, and PI3K/AKT signalling pathway | Dai et al. (2019) | |
| RNA-Seq | Capacitated sperm | miR-127, miR-1343, miR-151-3p | Calcium signalling pathway and MAPK signalling pathway | Li et al. (2018) | |
| RNA-Seq | Fresh vs frozen semen | miR-17-5p, miR-26a-5p, miR-486-5p, miR-122-5p, miR-184, and miR-20a-5p | Regulate PTEN, PI3K/AKT, and STAT signalling influence motility, viability, and sperm apoptosis | Dai et al. (2019) | |
| let-7a, -7d, -7e, miR-22, let-7d, and let-7e | Sperm motility | ||||
| miR-3155, miR-8197, miR-6727, miR-11796, miR-14189, miR-6125, and miR-13659 | Bovine sperm fertilization | ||||
| miRNAs, miR-26a, and miR-455-5p | Sperm motility | ||||
| miR-10a and miR-1 were | Sperm motility | ||||
| Microarray | High fertile vs Low fertile | miR-615 | Capacitation | Alvarez-Rodriguez et al. (2020) | |
| miR-221 | Wnt2, BDNF, CREB-related genes,PI3K-Akt and the estrogen signalling pathway |