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. 2021 Dec 9;12:783946. doi: 10.3389/fphar.2021.783946

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Copyright © 2021 Hou, Ding, Du, Yu, Zhou, Cui and Nie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MTECs underwent EMT in the mode of LLI culture. (A) HE staining of MTECs after 0, 2, 4, and 8 days’ (d) LLI culture with the arrowheads indicating the representative cells in each panel. Scale bar = 10 μm. (B) Representative graph of EMT markers (E-cadherin, Vimentin, and α-SMA) and Krt5 after 0, 2, 4, and 8 days’ LLI culture. (C–F) The statistical data of EMT markers and Krt5 in the mode of LLI culture. ^* p < .05, compared with ALI group (0 days), n = 4. (G–J) Real-time PCR results for EMT marker and Krt5 mRNAs, and β-actin was used as internal reference. ^* p < .05, compared with ALI (0 days), n = 4–5. Mann–Whitney U test was used to analyze the difference of the means for significance.