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. 2021 Dec 9;12:783946. doi: 10.3389/fphar.2021.783946

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Copyright © 2021 Hou, Ding, Du, Yu, Zhou, Cui and Nie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ribosome biogenesis was enhanced in the mode of the LLI culture. (A–B) Real-time PCR results of ribosome biogenesis–related RNAs (Rps15a, Rps19, Rpl14, and Rpl22 as well as 45, 28, 18, and 5.8S rRNA). ^* p < .05, ^** p < .01, ^*** p < .001, compared with ALI group, n = 3. Student’s t-test was used to analyze the difference of the means. (C–D) Ribosome biogenesis–related RNAs were significantly upregulated in basal cells in the mode of LLI culture. ^* p < .05, ^** p < .01, ^*** p < .001 compared with ALI group within same cell type, ^# p < .05, ^### p < .001, compared with the other cells in corresponding group. n = 3. One-way ANOVA followed by Bonferroni’s test was used to analyze the difference of the means for significance.