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Percentage of double-positive cells of three donors, stained with CD4-Nb1 or CD4-Nb2 (100 nM), or CD4-Nb3 or CD4-Nb4 (1,000 nM), compared to anti-CD4 antibody and negative control Nb (GFP-Nb, 1,000 nM).
| Frequency CD3+ CD4+ (%) | ||||
|---|---|---|---|---|
| c (nM) | Donor 1 | Donor 2 | Donor 3 | |
| Anti-CD4 antibody | ~1 | 33.7 | 27.0 | 24.3 |
| CD4-Nb1 | 100 | 30.5 | 29.2 | 22.7 |
| CD4-Nb2 | 100 | 33.8 | 25.6 | 18.4 |
| CD4-Nb3 | 1,000 | 35.5 | 26.5 | 20.4 |
| CD4-Nb4 | 1,000 | 33.8 | 26.9 | 23.9 |
| GFP-Nb | 1,000 | 1.4 | 0.3 | 1.0 |
