Anti-influenza virus activity and cytotoxicity of 2j and oseltamivir carboxylate (OSC).
| Compounds | EC50 (μM)a | CC50b | ||
|---|---|---|---|---|
| H1N1c | H3N2c | H3N2 E119Vc | ||
| 2j | 11.53 ± 2.69 | 7.65 ± 0.70 | >100d | >500 |
| OSC | 0.25 ± 0.081 | 0.43 ± 0.080 | >100d | >500 |
a
EC50 refers to the concentration required to achieve 50% protection against virus-induced cytopathic effect. All the assays were performed in quadruplicate and the results are reported as the mean ± SD.
b
CC50 refers to the concentration of 50% cellular toxicity.
c
A/Puerto Rico/8/1934; H3N2, A/Moscow/10/99; H3N2 E119V, and A/Moscow/10/99H3N2 background containing an E119V mutation in the neuraminidase gene.
d
At 100 μM, the inhibition rates were 24.6% for 2j and 44.1% for OSC, respectively.