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(A) Amplification profile of standards for CMV real-time PCR. Standard DNA at 107 (solid circles), 106 (open circles), 105 (solid squares), 104 (open squares), 103 (solid triangles), 102 (open triangles), and 10 (solid diamonds) copies/well in duplicate was amplified for 50 cycles. (B) Standard curve for CMV real-time PCR. Ct values were plotted against various numbers of copies of standard DNA. The slope was −3.54 cycles/log decade. The correlation coefficient was 0.988.
