FIGURE 5.

Fascin‐K471 acetylation is associated with ESCC patient survival. A, Representative IHC staining images of AcK471‐Fascin in paired ESCC tissues and adjacent normal tissues (n = 146). The staining scores are shown in the scatter diagram. Data are presented as mean ± SEM. The Student's t‐test was used for statistical analysis. B and C, Kaplan‐Meier survival analysis of ESCC patients with different expression levels of AcK471‐Fascin alone (B) or in combination with total Fascin (C). High expression of AcK471‐Fascin with concomitant low expression of total Fascin was significantly associated with the prolonged overall survival (OS) and disease‐free survival (DFS) of patients with ESCC. “Others” indicate patients concurrently expressing high or low levels of Fascin and AcK471‐Fascin. D, Representative IHC staining images of PCAF in ESCC tissues and Kaplan‐Meier analysis of OS and DFS of 268 patients with ESCC according to PCAF expression. Log‐rank test was used for survival analysis. Abbreviations: ESCC: esophageal squamous cell carcinoma; SEM: standard error of the mean; PCAF: P300/CBP‐associated factor; IHC: immunohistochemistry; OS: overall survival; DFS: disease‐free survival