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. 2021 Dec 9;12:788358. doi: 10.3389/fphar.2021.788358

FIGURE 2.

FIGURE 2

Anti-P. acnes mechanism of Cath-MH. (A) Agglutination of P. acnes ATCC 6919 by Cath-MH. P. acnes were incubated with BSA (a), Cath-MH (2× MICs) (b), and Cath-MH (2× MICs) plus equal volume of 0.2 mg/ml LPS (c) or 0.2 mg/ml LTA (d) for 30 min before being stained with Gram staining kit. (B) CD spectra of 50 μM Cath-MH in different solutions. (C) Suppression of LTA on the anti-P. acnes activity of Cath-MH. Cath-MH at the concentrations of 0.5×, 1× and 2× MICs were mixed with indicated concentrations of LTA for 30 min and then incubated with P. acnes for 72 h before its MIC was measured. (D) CLSM observation. P. acnes treated with sterile saline (a), or Cath-MH (2× MICs, b) for 30 min, respectively. (E) SEM observation. P. acnes treated with sterile saline (a), or Cath-MH (2× MICs, b) for 30 min, respectively. Data are expressed as mean ± SEM (n = 3). *p < 0.05, ***p < 0.001 were considered statistically significant compared with the control group without LTA.