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. 2021 Dec 9;15:789903. doi: 10.3389/fncel.2021.789903

FIGURE 2.

FIGURE 2

Co-staining of VGLUT1 and the mossy fiber-enriched protein synaptoporin in the human hippocampus. (A) Synaptoporin (SPO, magenta) and VGLUT1 (green) co-staining in a hippocampus section, areas of overlap appear in white (left). Individual channels are shown in the middle (SPO) and right (VGLUT1) column. Dashed squares (white) are shown in higher magnification in panels (B–D) as images with dashed frames. The yellow arrows mark the borders of the stratum lucidum, i.e., CA3. Scale bar, 300 μm. Zoom-in images of stratum lucidum (B), stratum radiatum (C), and CA4 (D) are displayed in the same manner as outlined in panel (A). Contrast adjustment was performed identically for panels (B–D). Insets at the lower right of each image represent MIPs of high-resolution z-stacks (z = 1.98 μm) in the respective layer, imaging parameters and contrast adjustments were kept constant. (B) White arrowheads in the inset mark non-co-localizing SPO puncta. Scale bar overview, 20 μm; inset, 2 μm. (C) Scale bar overview, 10 μm; inset, 2 μm. (D) White arrowheads in the inset mark perisomatic SPO puncta of a mossy cell in CA4. Scale bar overview, 10 μm; inset, 3 μm. Images in panels (A–D) are derived from case 1.