FIGURE 3.

Distribution of SPO at VGLUT1-positive synapses in CA3 and DG layers of a human hippocampus. (A–E) High-resolution z-stacks (z = 1.98 μm) were acquired in a hippocampus section co-stained for SPO and VGLUT1. A tilescan of the VGLUT1 channel served as a map to identify the layers and define the z-stacks. Overlay of SPO (magenta), VGLUT1 (green), and DAPI (blue) is shown in the left, individual channels are shown in the middle (SPO) and right (VGLUT1) column. White squares are shown as higher magnified insets at the lower right of each image. Imaging parameters and contrast adjustments were kept constant for all images. Scale bars overviews, 30 μm; scale bars insets, 2 μm. Images in panels (A–E) were derived from case 1.