. 2021 Mar 25;11(20):12074–12085. doi: 10.1039/d0ra09703a

Free amino acid composition of the Wuyi rock tea samples analyzed by HPLC (mg g⁻¹ dry weight)^a.

Tea samples	SX-L	SX-M	SX-S	QZ-L	QZ-M	QZ-S	DHP-L	DHP-M	DHP-S	RG-L	RG-M	RG-S	P
ASP	1.02 ± 0.08^A	0.78 ± 0.01^B	0.78 ± 0.03^B	0.95 ± 0.09^A	0.54 ± 0.03^C	0.54 ± 0.10^C	0.44 ± 0.00^CDE	0.41 ± 0.01^DE	0.36 ± 0.01^EF	0.49 ± 0.01^CD	0.35 ± 0.02^EF	0.29 ± 0.01^F	P < 0.001
GLU	0.53 ± 0.04^B	0.23 ± 0.00^DE	0.23 ± 0.04^DE	0.61 ± 0.08^A	0.15 ± 0.01^E	0.13 ± 0.02^E	0.27 ± 0.01^D	0.21 ± 0.00^DE	0.18 ± 0.01^DE	0.44 ± 0.07^C	0.14 ± 0.01^E	0.17 ± 0.05^DE
ASN	0.27 ± 0.02^AB	0.17 ± 0.01^BC	0.11 ± 0.04^C	0.38 ± 0.02^A	0.08 ± 0.01^C	0.07 ± 0.02^C	0.10 ± 0.00^C	0.09 ± 0.00^C	0.06 ± 0.00^C	0.08 ± 0.00^C	0.05 ± 0.00^C	0.05 ± 0.00^C
SER	0.30 ± 0.03^B	0.21 ± 0.01^BC	0.18 ± 0.02^C	0.44 ± 0.12^A	0.19 ± 0.01^BC	0.18 ± 0.04^C	0.26 ± 0.01^BC	0.23 ± 0.01^BC	0.21 ± 0.02^BC	0.29 ± 0.01^B	0.19 ± 0.01^BC	0.17 ± 0.00^C
GLN	0.15 ± 0.01^A	0.08 ± 0.00^B	0.06 ± 0.02^B	0.17 ± 0.02^A	0.08 ± 0.00^B	0.00 ± 0.00^E	0.07 ± 0.00^B	0.06 ± 0.00^B	0.05 ± 0.00^B	0.08 ± 0.01^BC	0.03 ± 0.00^CD	0.02 ± 0.02^D
HIS	ND	ND	ND	0.06 ± 0.00^A	ND	ND	0.03 ± 0.02^B	0.02 ± 0.02^BC	ND	0.03 ± 0.00^B	ND	ND	P = 0.0395
GLY	0.11 ± 0.01^C	0.10 ± 0.00^C	0.10 ± 0.00^C	0.19 ± 0.04^A	0.11 ± 0.00^C	0.11 ± 0.01^C	0.16 ± 0.00^B	0.15 ± 0.00^B	0.15 ± 0.01^B	0.15 ± 0.01^B	0.13 ± 0.00^BC	0.14 ± 0.01^B	P < 0.001
THR	0.22 ± 0.02^A	0.16 ± 0.00^B	0.15 ± 0.01^B	0.21 ± 0.03^A	0.10 ± 0.01^DE	0.09 ± 0.03^E	0.12 ± 0.01^CD	0.10 ± 0.00^ED	0.09 ± 0.00^E	0.14 ± 0.01^BC	0.08 ± 0.00^E	0.07 ± 0.01^E
ARG	0.25 ± 0.03^B	0.17 ± 0.02^CD	0.14 ± 0.03^D	0.49 ± 0.10^A	0.26 ± 0.03^B	0.23 ± 0.04^BC	0.12 ± 0.00^D	0.13 ± 0.00^D	0.09 ± 0.00^D	0.14 ± 0.01^D	0.09 ± 0.01^D	0.09 ± 0.01^D
ALA	0.51 ± 0.01^D	0.52 ± 0.01^D	0.57 ± 0.02^CD	0.85 ± 0.07^A	0.67 ± 0.00^B	0.70 ± 0.04^B	0.37 ± 0.02^F	0.41 ± 0.01^EF	0.44 ± 0.01^E	0.62 ± 0.02^C	0.54 ± 0.01^D	0.55 ± 0.01^D
Theanine	5.02 ± 0.39^B	2.35 ± 0.03^CD	1.96 ± 0.22^E	5.49 ± 0.30^A	1.38 ± 0.10^F	1.08 ± 0.24^FG	2.61 ± 0.00^C	1.89 ± 0.04^E	1.26 ± 0.01^F	2.24 ± 0.07^DE	0.81 ± 0.05^G	0.51 ± 0.00^HI
TYR	0.51 ± 0.04^A	0.39 ± 0.00^C	0.43 ± 0.03^B	0.33 ± 0.05^D	0.16 ± 0.01^G	0.16 ± 0.03^G	0.26 ± 0.00^E	0.24 ± 0.01^EF	0.21 ± 0.01^FG	0.16 ± 0.01^G	0.10 ± 0.01^H	0.09 ± 0.00^H
VAL	0.31 ± 0.03^A	0.23 ± 0.00^B	0.22 ± 0.00^B	0.29 ± 0.07^A	0.15 ± 0.01^CD	0.16 ± 0.03^CD	0.20 ± 0.00^BC	0.17 ± 0.00^CD	0.15 ± 0.00^CD	0.17 ± 0.01^CD	0.12 ± 0.01^D	0.13 ± 0.03^D
MET	0.10 ± 0.00^E	0.11 ± 0.00^D	0.12 ± 0.00^C	0.14 ± 0.00^B	0.15 ± 0.00^A	0.16 ± 0.01^A	ND	ND	ND	ND	ND	ND
TRP	0.42 ± 0.03^A	0.28 ± 0.01^B	0.29 ± 0.02^B	0.41 ± 0.07^A	0.17 ± 0.01^C	0.18 ± 0.00^C	0.19 ± 0.00^C	0.16 ± 0.00^C	0.14 ± 0.00^CD	0.19 ± 0.00^C	0.11 ± 0.01^DE	0.08 ± 0.00^E
PHE	0.21 ± 0.02^A	0.15 ± 0.00^BC	0.15 ± 0.01^BC	0.16 ± 0.03^BC	0.06 ± 0.00^D	0.06 ± 0.01^D	0.20 ± 0.00^A	0.17 ± 0.00^B	0.14 ± 0.00^C	0.14 ± 0.01^C	0.08 ± 0.01^D	0.07 ± 0.01^D
ILE	0.17 ± 0.01^A	0.12 ± 0.00^BC	0.10 ± 0.01^CD	0.14 ± 0.05^B	0.05 ± 0.00^E	0.05 ± 0.01^E	0.07 ± 0.00^DE	0.06 ± 0.00^E	0.05 ± 0.00^E	0.07 ± 0.01^E	0.04 ± 0.00^E	0.04 ± 0.01^E
LEU	0.11 ± 0.01^B	0.13 ± 0.00^A	0.14 ± 0.01^A	0.10 ± 0.00^B	0.11 ± 0.01^B	0.11 ± 0.02^B	0.07 ± 0.00^C	0.06 ± 0.00^CD	0.05 ± 0.00^CD	0.06 ± 0.00^DE	0.04 ± 0.00^E	0.04 ± 0.01^E
LYS	0.62 ± 0.05^A	0.43 ± 0.01^B	0.35 ± 0.06^C	0.45 ± 0.13^B	0.19 ± 0.01^D	0.18 ± 0.03^DE	0.14 ± 0.00^DE	0.12 ± 0.00^DE	0.10 ± 0.01^DE	0.11 ± 0.01^DE	0.07 ± 0.00^E	0.06 ± 0.01^E
Total	10.8 ± 0.8^B	6.6 ± 0.1^C	6.1 ± 0.2^C	11.8 ± 1.5^A	4.6 ± 0.2^DE	4.2 ± 0.7^E	5.7 ± 0.0^CD	4.7 ± 0.1^DE	3.7 ± 0.1^EF	5.6 ± 0.3^CD	3.0 ± 0.2^FG	2.6 ± 0.2^G

ND: not detected. Data are expressed as mean ± SD (n = 3). Values in a row followed by different letters are significantly different (P < 0.05). Data marked with different superscripted letters are significantly different at P < 0.05 level by the S–N–K test using SPSS 19.0 software. Capital letters (A, B, C…) represent a statistically significant difference within each row.

Free amino acid composition of the Wuyi rock tea samples analyzed by HPLC (mg g−1 dry weight)a.

Free amino acid composition of the Wuyi rock tea samples analyzed by HPLC (mg g⁻¹ dry weight)^a.