Identification and physicochemical properties of ACEI peptides from P. fucata protein by LC-ESI-MS/MS and in silico analysis^a.

Peptide	m/z	Fraction	ALC (%)	P-Value	Toxicity	Net charge	Hydrophobicity	Isoelectric point	Reported ACEI peptides
FRVW	607.3314	C17	98	0.0008755	None	+1	−0.06	pH 10.59	VW, FR
VGPGW	515.2564	C17	88	0.001274	None	0	0.23	pH 3.57	GP, GW, VG, PG, VGP
LPYY	555.2881	C18	87	0.00124	None	0	0.12	pH 3.57	—
GTPW	460.2251	C18	86	0.0007006	None	0	0.07	pH 3.6	GT, TP
FGFWT	657.2963	C17	86	0.004784	None	0	0.31	pH 3.44	GF, FG
RFTGW	666.3347	C17	86	0.0006071	None	+1	−0.16	pH 10.55	RF, GW, TG
WAPR	529.2845	C17	83	5.122 × 10−5	None	+1	−0.3	pH 10.72	PR, AP, WA
RWPN	572.2934	C18	82	7.924 × 10−5	None	+1	−0.53	pH 10.55	RW
WDDM	566.2004	C17	82	0.01177	None	−2	−0.2	pH 0.62	DM
NWLN	546.2726	C18	81	0.008376	None	0	−0.1	pH 3.29	LN, WL
WELSKS	749.3749	C18	81	0.01114	None	0	−0.22	pH 6.73	—
TVTW	506.2704	C17	80	0.00243	None	0	0.14	pH 3.29	—
PAAPW	541.2823	C17	80	0.0001537	None	0	0.15	pH 4.04	AAP, AP, AA

^aAbbreviation: ALC, average local confidence.