FIG. 5.
Expression of Ink4 proteins and Cdks during testis development in wild-type mice. (A) Lysates from whole testes isolated from wild-type males at 7, 9, 11, 13, 15, 17, 19, and 25 days pp and at 1 and 5 months (m) of age were normalized for protein content and precipitated with antibodies to Ink4 proteins or Cdks. Denatured precipitates were electrophoretically separated on denaturing gels and immunoblotted with antisera directed to the same proteins, as indicated at the left of the panels. (B) Lysates were precipitated with antibodies directed to either p18Ink4c or p19Ink4d, as indicated at the top of the panels. Immunoprecipitates (IP) were denatured, separated electrophoretically, and immunoblotted with antisera to the Cdks noted at the left of the panels. Controls performed with cells of Ink4cd double-null mice are in lanes C.