Table 2.
Details on SARS-CoV-2 proteins.
| Serology Assays | |||
|---|---|---|---|
| Protein | SARS-CoV-2 Sequence Length (AA) | Backbone | Expression |
| Full-length Spike (with D614G) | 1195 (14-1208) | Wuhan WT spike | CHO |
| Spike-RBD | 199 (329-527) | Wuhan RBD | CHO |
| Spike-RBD E484Q | 223 (319-541) | Wuhan RBD | CHO |
| Spike-NTD | 294 (14-307) | Wuhan NTD | CHO |
| NCP | 419 (1-419) | Wuhan NCP | CHO |
| Pseudovirus Assays | |||
| E484Q | 1256 (1-1256) | Wuhan spike | NA |
| E484K | 1256 (1-1256) | Wuhan spike | NA |
| B.1.351 | 1253 (1-241; 245-1256) | Wuhan spike with (L18F,D80A,D215G,del242-244, K417N,E484K, N501Y,D614G, A701V) | NA |
CHO, Chinese hamster ovary cells; NA, not applicable; NCP, nucleocapsid protein; NTD, N-terminal domain; RBD, receptor-binding domain; WT, wild-type.