TABLE 2.

Conserved PCR primer designations and subtype-specific restriction enzymes

Gene	Forward primer	Reverse primer	Amplicon size (bp)	Subtype-specific restriction enzyme
				H1N1	H3N2	H5N1
NS	1	890	890	DraI	XbaI	BsrBI
M	149	847	698	HindIII	ScaI	AvaII
NP	528	1506	978	HaeII	SacII	BamHI
PA	29	773	744	BbsI	EcoNI	NsiI
PB1	22	715	693	ScaI	XmnI	BsrBI
PB2	160	1007	847	EcoRV	BstZ171	BglII