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. 2021 Dec 23;18:299. doi: 10.1186/s12974-021-02365-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Macrophage targeted two-color (DiI/DiR) fluorescent nanoemulsion. A PBS-based PFC-NE particle size distribution overlay comparison between day 1 and 8 months after manufacturing. B pH stability of PFC-NE measured upon manufacture and up to 21 days. C PFC-NE serum stability in three different biological media (Water, DMEM, and 20% FBS in DMEM) was measured at time 0 and after 72 h incubation at elevated temperature of 37 °C (p = 0.514, p = 0.777, p = 0.969 respectively, Student’s T-test, n = 3). D PFC-NE centrifugation stability was conducted with 3000 rpm for 30 min. PFC-NE (undiluted) size was measured before and after centrifugation (p = 0.097, Student’s T-test, for n = 3). E PFC-NE stability was tested and analyzed before and after sterile-filtration with a 0.22 μm MCE membrane filter. All particle size and/or distribution were measured by Dynamic light scattering (DLS) (p = 0.642, Student’s T-test, n = 3). F Fluorescence signals of the PFC-NE with NIRF dye DiR were compared between week 1 and week 20 after manufacturing. PFC-NE was diluted at PFC-NE to water ratio of 1:4. The fluorescence signal was collected by LI-COR Odyssey Imager. Error bar represents the standard deviation from three independent measurements