Fig. 3.

Nanoemulsion uptake and impact on macrophage viability in vitro. PBS-based PFC-NE uptake when exposed to RAW 264.7 (ATCC TIB 71) macrophage cell line. Microscopy images were taken on EVOS Imager at 40X Magnification. White bar on the lower right corner represents 100 µm A Image of macrophage morphology when exposed to PFC-NE under the White Light channel. B Image of macrophages’ nuclei when exposed to PFC-NE, and treated with Hoechst stain under the DAPI channel. Cell nucleus can be observed in blue. C Image of macrophages’ cytoplasm when exposed to NE under the Red Fluorescent Channel. D Macrophage cell viability upon 24 h exposure of NE with 10 µM and 25 µM of Near infrared (NIRF) dye DiR concentration. Cellular viability was assessed using CellTiter-Glo^® 2.0. The error bar represents the standard deviation from six independent measurements