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. 2021 Dec 22;20:240. doi: 10.1186/s12933-021-01426-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Pre-stroke T2D and dietary change leading to weight loss do not significantly alter vessel density after tMCAO. a CD31+ vessel density in contralateral striatum and infarct core (ipsilateral striatum) after tMCAO. b CD31+ vessel density in the infarct border (ipsilateral striatum) after tMCAO. Data are presented as mean ± SD. Two-way ANOVA (a) or Brown–Forsythe and Welch ANOVA (b) followed by two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli was used for analyses. * Denotes p < 0.05. c Representative images of CD31+ vessel staining in the ipsilateral striatum of non-T2D, T2D/Ob and T2D/WL mice after tMCAO. White solid lines indicate the infarct region (infarct region is on right side of the solid line; peri-infarct region is on left side of the solid line). White dotted lines delineate the infarct border region that was analyzed in figure (b). Scale bar: 100 µm. Group sizes: a, b non-T2D n = 7, T2D/Ob n = 6, T2D/WL n = 6