Fig. 5.

Luciferase activity measured in the producer and infected cells supplemented with viral protease plasmid. HEK 293 T cells were co-transfected with 2 µg of pNL4.3 and 2 µg of GFP(+) ZIKV. Increasing amounts (1–5 µg) of the NS2B/NS3 protease plasmid were added. Luciferase activity measured in (a) infected and (b) producer cells showed no infectious pseudotypes produced and no reduction in luciferase activity in the producer cells. (c) Murine Leukaemia Virus (MLV) backbone was tested at 2 µg with 2 µg GFP(+) ZIKV to identify if the backbone had an effect on infectivity. No infectious particles were produced. VSV-G and Δ envelope were used as positive and negative controls, respectively. Graphs show mean±sd of three independent experiments.