Fig. 4. RT-PCR with sequence visualisation (A–C) and densitometry analysis (D) of RNA prepared from H2K mdx mouse myotubes transfected with ASO11 (A), 12 (B) and control 2′-MOE (C). The triangles above the gel image indicate increasing ASO concentration (2.5, 5, 10, 25, 50 and 100 nM); FL (full-length) = 901 bp, represented in grey color; Δ23 (exon-23 skipped) = 688 bp, represented in blue color; Δ22–23 (exons-22 + 23 skipped) = 542 bp, represented in green color; UT = untreated, Ne = negative control.
