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. 2021 Dec 20;9:e12595. doi: 10.7717/peerj.12595

Figure 1. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) express the Sigma-1 receptor (S1R).

Figure 1

(A) End point PCR analysis of S1R on mRNA isolated from three hiPSC-CMs replicate samples from three independent batches of differentiation (N = 3). GAPDH was used as housekeeping control of mRNA samples. Uncropped agarose gel is appended in Fig. S5 with appropriate controls. (B) Representative western blot of S1R expression in protein extracts of control hiPSC-CMs. Actin was used as a loading control. The detection of the protein was performed in samples from 3 different batches with similar results (N = 3). Full-length gels of the representative blots shown here are presented in Fig. S5. (C) Representative image of staining performed in fixed cardiomyocytes from three different batches shows S1R presence and similar distribution in hiPSC-CMs (N = 3). S1R (green); phalloidin (red) and nuclei (blue); scale bar = 50 µm. (D) Immunofluorescence shows mitochondrial marker VDAC1/Porin (red) and S1R (green) in hiPSC-CMs. Representative image of three independent experiments (N = 3). Nuclei were stained with DAPI (blue); scale bar = 50 µm. (E) Immunofluorescence shows ER chaperone lectin Calnexin (CNX) (red) and S1R (green) in hiPSC-CMs. Representative image of 3 independent experiments (N = 3). Nuclei were stained with DAPI (blue); scale bar = 50 µm. Zoom-in highlights the cells indicated by the arrowheads (D and E).