Skip to main content
. 2021 Dec 20;9:e12595. doi: 10.7717/peerj.12595

Figure 4. NE-100 does not induce death and ER stress but decreases beating frequency in hiPSC-CMs.

Figure 4

(A) Neutral red cell viability assay for escalating NE-100 concentrations shows non-significant changes after 72 h post-treatment. Data are represented as the baseline-corrected mean ± S.E.M relative to the vehicle condition analyzed by one-way ANOVA (N = 3). (B) Nuclear size analysis by DAPI staining shows the percentage of pyknotic nuclei after 48 h (N = 3); Dots represent the percentage of each well evaluated in three independent experiments analyzed by unpaired Welch’s t test; non-significant (p = 0.1738). (C) Normalized mRNA expression of spliced and unspliced XBP1 transcripts in samples from different batches exposed to NE-100 1 µM in four independent experiments (N = 4). Data are expressed relative to total XBP1 normalized expression ± S.E.M and were analyzed by multiple t-tests (Holm-Sidak method) (spliced/total p = 0.7338 and unspliced/total p = 0.6943). (D) CHOP mRNA in samples from different batches exposed to NE-100 1 µM in three independent experiments (N = 3). Values are expressed as fold-change relative to the untreated condition ± S.E.M and were analyzed by unpaired Welch’s t test; non-significant (p = 0.5512). (E) Average of beats per minute analyzed 24 and 48 h after exposure to NE-100 or vehicle (N = 3). Data are presented as the average ± S.E.M, statistical differences were analyzed by multiple t-tests (Holm-Sidak method) (24 h p = 0.03663; 48 h p = 0.00349).