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. 2000 Jul;38(7):2611–2621. doi: 10.1128/jcm.38.7.2611-2621.2000

FIG. 1.

FIG. 1

(A) Immunoblots with dog sera using different Borrelia strains as sources of antigens. Lane 1, negative-control dog; lane 2, asymptomatic dog 35; lane 3, symptomatic dog 33; lane 4, symptomatic dog 48; lane 5, positive-control dog. The molecular masses of the proteins were assessed by running a prestained low-molecular-mass marker adjacent to the cell lysate. All three strains were tested for the presence of OspA, OspB, OspC, and flagellin with MAb. (B) Immunoblots with dog sera using strains A87S and B31 as sources of antigens. Lane 1, negative-control dog; lane 2, asymptomatic dog 57; lanes 3 and 4, preclinical sera from dog 33; lane 5, acute-phase serum from dog 33; lane 6, chronic-phase serum from dog 33; lanes 7 and 10, preclinical serum from dog 28; lanes 8 and 11, acute-phase serum from dog 28; lanes 9 and 12, convalescent serum from dog 28. Identification of the proteins was as for panel A.