TABLE 3.

Serum antibodies against strains of B. burgdorferi sensu lato as detected on immunoblots, serum bactericidal activity, and DNA detection by PCR in dogs

Dog or serum	Agea (yr)	Strainsi for which antibodies against bands with the indicated molecular masses (kDa) were detected																					Bactericidal activity againste	DNA detection by PCRf
		75	66	62	60	58	56	54	50	48	45	43	41 (Fla)	39	33–34b (OspB)	31c (OspA)	30	28	26	25	21d (OspC)	<19
Symptomatic dogs
28	4	g	bga	g	a	bg	a	a	g	g	a	a	bga	bga	ba	bga	b	b	ga		g	ga	None	NDj
47	6	g	bg	g	a	bg	a		g	g	a		bga	bga	bga	ba			a		a	a	None	b
63	7		bga	g		ga	ba	b	g	g		a	bga	bg	ba	g	b	bg			g		None	ND
14	2		a		g	a	b				b		bga	bga	a			b			g		g	bg
40	2	ga	bg	g	a	bg	a	a	g	g		a	bga	ga	ba		b				bg	g	g	ND
58	4	bg	bga	g	a	bg	a		g	g	a	a	bga	bga	ga	a			a		g	a	g	bgav
72	8		b	g					g		a	a	bga	b	b								bg	ND
107	8	g	bga	b	ba	bg	ba	a			ba	a	ba	ga	ga	a	b			a		bga	None	b
48	2	bga	bga	bga	bga	g	ba	a	a	g	ga	a	bg	ba	ba	a	b	bg	a	ba	ba	bga	a	ND
33	4	bga	bga	ga	b	g	ba	a	a			a	bg	b	bga	a	b	bg	a	a	ba	ga	a	ND
75	9		b										ga		a								None	Negative
77	9			a			a	a				a	bga	bga	a				a	a			None	ND
79	12						a						bga	b	a								b	b
78	13			b									b										None	b
83	13									g		a	ga	ga	a	a							None	bga
Asymptomatic dogs
501	2																						None	g
10	3		b										bg	bg									None	ND
22	3		g			b							bg	g				g					ND	ND
34	5		b			b							bga	bg	bg			b					ND	ND
35	5									g		a	bga	bga									None	ND
39	5		g		ba					g			bga	ga									None	ND
54	7		bga			b							bga	ga	a								None	ND
57	8		bg							g		a	bga	bga	b			g		g			None	ND
59	8		b									a	bga	ba									None	ND
94	8		g										ba	bga									None	v
62	10						a						bga	bga	a								None	ND
67	10												bga	g	a								None	ND
68	11		g										bga		bg								ND	ND
76	13					b							bga	bga	ga			g					ND	Negative
511	13		b										bg	g	g								None	a
Positive control
A92 1/2g	1		bga			g	a	ba		g	ga	a	bga	ba	ba		b	bg	a	a		bg
Negative controlh
Lep. vac.		a				g							ba
NCS (n = 1)													bga
NCS (n = 3)

^aAs defined for Table 1. 

^bStrains B31 and pKo expressed a 34-kDa protein, and strain A87S expressed a 33-kDa protein, both of which reacted with MAb for OspB. 

^cStrains B31 and A87S expressed a 31-kDa protein that reacted with MAb for OspA, whereas strain pKo expressed a 31-kDa protein that did not react with the MAb for OspA. 

^dStrains A87S and pKo expressed a 21-kDa protein that reacted with the MAb for OspC, whereas strain B31 expressed a 21-kDa protein that showed no reactivity to the OspC MAb. 

^eResults of immobilization assays. See footnote i for strain abbreviations. None, no immobilization found. 

^fFor some dogs, tissue biopsies for the detection of specific Borrelia DNA, which was determined with species-specific probes, were available. b, B. burgdorferi sensu stricto; g, B. garinii; a, B. afzelii; v, B. valaisiana. 

^gAs defined for Table 1. 

^hAs defined for Table 1. 

ⁱb, B. burgdorferi sensu stricto strain B31; g, B. garinii strain A87S; a, B. afzelii strain pKo. 

^jND, not done.