Table 2.
Panel of porcine oligonucleotide primers used for real-time PCR.
| Target Gene | Accession No. | Forward Primer (5’-3’) Reverse Primer (5’-3’) |
Amplicon Length (bp) |
|---|---|---|---|
| IL6 | NM_214399.1 | F: GACAAAGCCACCACCCCTAA R: CTCGTTCTGTGACTGCAGCTTATC |
69 |
| CXCL8 | NM_213867.1 | F: TGCACTTACTCTTGCCAGAACTG R: CAAACTGGCTGTTGCCTTCTT |
82 |
| IL10 | NM_214041.1 | F: GCCTTCGGCCCAGTGAA R: AGAGACCCGGTCAGCAACAA |
71 |
| IL17A | NM_001005729.1 | F: CCCTGTCACTGCTGCTTCTG R: TCATGATTCCCGCCTTCAC |
57 |
| IFNG | NM_213948.1 | F: TCTAACCTAAGAAAGCGGAAGAGAA R: TTGCAGGCAGGATGACAATTA |
81 |
| TNF | NM_214022.1 | F: TGGCCCCTTGAGCATCA R: CGGGCTTATCTGAGGTTTGAGA |
68 |
| TGFB1 | NM_214015.1 | F: AGGGCTACCATGCCAATTTCT R: CGGGTTGTGCTGGTTGTACA |
101 |
| TLR4 | NM_001293317.1 | F: TGCATGGAGCTGAATTTCTACAA R: GATAAATCCAGCACCTGCAGTTC |
140 |
| MUC1 | XM_001926883.1 | F: ACACCCATGGGCGCTATGT R: GCCTGCAGAAACCTGCTCAT |
68 |
| MUC2 | XM_021082584.1 | F: CAACGGCCTCTCCTTCTCTGT R: GCCACACTGGCCCTTTGT |
70 |
| ZOI | XM_005659811.1 | F: TGAGAGCCAACCATGTCTTGAA R: CTCAGACCCGGCTCTCTGTCT |
76 |
| CLND3 | NM_001160075.1 | F: GAGGGCCTGTGGATGAACTG R: GAGTCGTACACTTTGCACTGCAT |
65 |
| CCK | NM_214237.2 | F: GGACCCCAGCCACAGAATAA R: GCGCCGGCCAAAATC |
|
| FABP2 | NM_001031780.1 | F: CAGCCTCGCAGACGGAACTGAA R: GTGTTCTGGGCTGTGCTCCAAGA |
102 |
| SLC2A1/GLUT1 | XM_003482115.1 | F: TGCTCATCAACCGCAATGA R: GTTCCGCGCAGCTTCTTC |
72 |
| SLC2A2/GLUT2 | NM_001097417.1 | F: CCAGGCCCCATCCCCTGGTT R: GCGGGTCCAGTTGCTGAATGC |
96 |
| SLC2A5/GLUT5 | XM_021095282.1 | F: CCCAGGAGCCGGTCAAG R: TCAGCGTCGCCAAAGCA |
60 |
| SLC5A1/ SGLT1 | NM_001164021 | F: GGCTGGACGAAGTATGGTG R: ACAACCACCCAAATCAGAGC |
|
| SLC15A1/PEPT1 | NM_214347.1 | F: GGATAGCCTGTACCCCAAGCT R: CATCCTCCACGTGCTTCTTGA |
73 |
| VDR | NM_001097414.1 | F: CCTTCACCATGGACGACATG R:TGGCCACGTCGCTGACTT |
73 |
| B2M | NM_213978.1 | F: CGGAAAGCCAAATTACCTGAAC R:TCTCCCCGTTTTTCAGCAAAT |
83 |
| GAPDH | NM_001206359 | F: CAGCAATGCCTCCTGTACCA R: ACGATGCCGAAGTTGTCATG |
72 |
| PPIA | NM_214353.1 | F: CGGGTCCTGGCATCTTGT R: TGGCAGTGCAAATGAAAAACT |
75 |
| YWZHAZ | NM_001315726.1 | F: GGACATCGGATACCCAAGGA R:AAGTTGGAAGGCCGGTTAATTT |
71 |