Figure 4.

In vivo gastrointestinal permeability of broiler chickens infected with different dosages of Eimeria tenella. In vivo gastrointestinal permeability was measured using fluorescein isothiocyanate dextran 4 kDa in the T0 (treatment 0; Sham-challenged with phosphate-buffered saline); T1 (treatment 1; challenged with 6250 sporulated oocysts of E. tenella); T2 (treatment 2; challenged with 12,500 sporulated oocysts of E. tenella); T3 (treatment 3; challenged with 25,000 sporulated oocysts of E. tenella); T4 (treatment 4; challenged with 50,000 sporulated oocysts of E. tenella) groups on 5, 6, and 7 days post-infection. At each time point, orthogonal polynomial contrasts analysis was conducted to see linear pattern (L) and quadratic pattern (Q) among the treatments.