Table 2.
Primer sequences and product lengths of target gene fragments.
| Transcripts | Sequence (5′–3′) | Product Size (bp) | |
|---|---|---|---|
| Oxidative | Nrf2 | F: GGCCGCCTTGAAGCTCATCTC | 177 |
| stress | R: CTTGCCTCTCCTGCGTATATCTCG | ||
| Keap1 | F: TTTTCAAGACCTCACCCTCCATAAACC | 113 | |
| R: AGTAGCCCAAGGACTGCCGATAG | |||
| HO-1 | F: AGAGCCAGGAGAACGGTCACC | 114 | |
| R: TGCTTGTCCTCTCGCTTTCTTTCC | |||
| CAT | F: ATGGACCAATGTGCGTGACTGAC | 104 | |
| R: CATGCGGCTCTCCTTCACAACAG | |||
| SOD1 | F:TCTCTCTGACTGGACCACACTGC | 100 | |
| R: GTTAGCGTGCTCTCGTTGTCTCC | |||
| NQO1 | F: GCTCTCACGGCACGACATCAC | 84 | |
| R: TCCTTCCACGCTTCTCCCATCTC | |||
| GST | F: GACTTTCTTGTGGGGAACCGACTC | 102 | |
| R: AGGAAACCCAGAAAGCACAGCAG | |||
| GCLM | F: TGTTGTGTGATGCCACCTGATCTC | 150 | |
| R: CCATTCGTGTGCTTTGACGTTCTG | |||
| GCLC | F: TTCAGGTGACATTCCAGGCTTGC | 108 | |
| R: AGAACGGAGATGCAGCACTCAATG | |||
| Detoxification | CYP1A1 | F: AGGACGGAGGCTGACAAGGTG | 104 |
| R: AGGATGGTGGTGAGGAAGAGGAAG | |||
| CYP1A4 | F: AATGCTCGTTTCAGTGCCTTC | 127 | |
| R: CCTCCCCTGTCCTTTTCTCC | |||
| CYP3A4 | F: GACGTGCAGAAGCGACTCCAG | 161 | |
| R: TTCTTGCAGATCCGCTCAATCCG | |||
| Inflammation | IL-10 | F: CAACCTGCTGCTGAGCCTGAAG | 133 |
| R: CGCCTTGTAGATGCCGTTCTCG | |||
| IL-16 | F: TCAACGGACTCGCAGTAACTCAAC | 95 | |
| R: GATCTCTGAAGGGCACGGCTTTC | |||
| IL-18 | F: ACCCTCCATCGCTTCCTTCGG | 113 | |
| R: CCGCTGCCAGATTTCATCTCCTG | |||
| TNF-α | F: CCGTGGTCAGTTTCCATCAGG | 117 | |
| R: ACTTTGCAGTTAGGTGACGCT | |||
| NF-κB | F: GGAGCAGTGGCGGTGTCAAC | 126 | |
| R: AGTGCAGTTCATGTCATCGGTCTC | |||
| Sirt1 | F: TGACAGAGCCTCACATGCAAGTTC | 144 | |
| R: GGTGGAGGGATTGTTTCTGGTAGC | |||
| Apoptosis | Caspase3 | F: TGAGGCAGACAGTGGACCAGATG | 110 |
| R: CTGCATTCCGCCAGGAGTAATAGC | |||
| Caspase9 | F: TGGATTGCGATTCACCCGAAGATG | 83 | |
| R: ATTACCCGAGGGAGCCTGGAAAG | |||
| Bax | F: GCGGACGGAGCCTTCAACTG | 84 | |
| R: CTGCGAGAACAGAGCCTTGATGG | |||
| Bcl-2 | F: GAGTTCGGCGGCGTCATGTG | 155 | |
| R: CCATACAACTCCACGAAGGCATCC | |||
| P53 | F: ACTGCTACGTCGCGGCTCTC | 199 | |
| R: CGCTGGCAAGGCTGGTGAAC | |||
| Internal | β-actin | F: ATGTCGCCCTGGATTTCG | 62 |
| reference | R: ATGTCGCCCTGGATTTCG |
Nrf2, nuclear factor erythroid 2-related factor 2; Keap1, Kelch-like ECH-associated protein; HO-1, heme oxygenase 1; CAT, catalase; SOD, superoxide dismutase; NQO1, NAD(P)H quinone oxidoreductase 1; GCLC, glutamate cysteine ligase catalyzes subunits; GCLM, glutamic acid cysteine ligase modified subunit; Caspase-1, cysteine-dependent aspartate-directed protease-1; CYP1A1, cytochrome P450 1A1; CYP1A4, cytochrome P450 1A4; CYP3A4, cytochrome P450 3A4; IL-10, interleukin-10; IL-16, interleukin-16; IL-18, interleukin- 18; TNF-α, tumor necrosis factor α; NF-κB, nuclear factor-κb; Sirt1, Sirtuin 1; Caspase-3, cysteine-dependent aspartate-directed protease-3; Caspase-9, cysteine-dependent aspartate-directed protease-9; Bax, Bcl-2 associated X; Bcl-2, Mus musculus B cell leukemia/lymphoma 2.