TABLE 1.

Comparison of five PCR assays for detection of C. pneumoniae DNA

Assay	Target	Assay format	Primers	Amplicon size (bp)	No. of amplification cycles	Reference
1	Cloned PstI	Standard	HL1 5′ GTT GTT CAT GAA GGC CTA CT 3′	437	40	7
			HR1 5′ TGC ATA ACC TAC GGT GTG TT 3′
2	16S rRNA	Standard	CpnA 5′ TGA CAA CTG TAG AAA TAC AGC 3′	463	40	13
			CpnB 5′CGC CTC TCT CCT ATA AAT 3′
3	16S rRNA	Nested	CpnA 5′ TGA CAA CTG TAG AAA TAC AGC 3′	463	40	14
			CpnB 5′ CGC CTC TCT CCT ATA AAT 3′
			TW50 5′ AGT CCC GCA ACG AGC GCA 3′	270	30
			TW51 5′ GCT GAC ACG CCA TTA CTA 3′
4	ompA	Nested	CP1 5′ TTA CAA GCC TTG CCT GTA GG 3′	333	40	36
			CP2 5′ GCG ATC CCA AAT GTT TAA GGC 3′
			CPC 5′ TTA TTA ATT GAT GGT ACA ATA 3′	207	30
			CPD 5′ ATC TAC GGC AGT AGT ATA GTT 3′
5	16S rRNA	TETR	CPN90 5′ GGT CTC AAC CCC ATC CGT GTC GG 3′	197	60	21
			CPN91 5′ TGC GGA AAG CTG TAT TTC TAC AGT T 3′