TABLE 3.

Reproducibility of five PCR assays for detecting C. pneumoniae^a

No. of IFU	No. of aliquots testing positive for PCR assay:
	1	2	3	4	5
0.01	10	10	10	10	10
0.005	5	6	6	8	10
0.002	3	4	5	6	4
0.001	4	4	4	7	9
0.0004	1	3	6	5	4
0.0001	0	0	0	0	0
Total	23	27	31	36	37

^aTen replicate aliquots of a serial dilution of C. pneumoniae DNA were tested by each of five PCR assays described in Table 1. DNA was purified from a 1-ml stock C. pneumoniae strain VR1310 as described in the footnote for Table 2.