Table 2.

General information about the target and reference genes.

Category	Gen/Protein Description	Abbrev.	Primer Sequence		GenBank	BP Size	Reference
			Forward	Reverse
Housekeeping genes	Beta-actin	β-actin	GCCGGCCGCGACCTCACAGACTAC	CGGCCGTGGTGGTGAAGCTGTAAC	EZ908974	73	Evenhuis and Cleveland, [58]
	Elongation factor 1-alpha	ELF-1α	ACCCTCCTCTTGGTCGTTTC	TGATGACACCAACAGCAACA	AF498320	63	Kania et al. [59]
	Transferrin		5’CCACCTCCAGGGCCATTAAATG3’	5’ATCCACCGCTATGGCATCTGCC3’	D89083		Talbot et al. [60]
Immune	Immunoglobulin T	IgT	AACATCACCTGGCACATCAA	TTCAGGTTGCCCTTTGATTC	AY870265	80	Evenhuis and Cleveland, [58]
	Interferon gamma	IFN-γ	CTGTTCAACGGAAACCCTGT	AACACCCTCCGATCACTGTC	NM 001160503	62	Evenhuis and Cleveland, [58]
	Interleukin-1 beta	IL-1β	ACATTGCCAACCTCATCATCG	TTGAGCAGGTCCTTGTCCTTG	AJ223954	91	Pérez-Sánchez et al. [61]
Inflammatory	Interleukin 8	IL-8	CTCGCAACTGGACTGACAAA	TGGCTGACATTCTGATGCTC	AJ279069	148	Evenhuis and Cleveland, [58]
	Transforming growth factor beta	TGF-β	TCCGCTTCAAAATATCAGGG	TGATGGCATTTTCATGGCTA	X99303	71	Evenhuis and Cleveland, [58]