Figure 7.

LPS-induced alterations in the expression of cell signaling proteins. Protein extracts from Rin-5F cells treated with LPS with/without AZD were separated on 12% SDS-PAGE and transferred on to nitrocellulose paper by Western blotting. Specific antibodies against AMPK/p-AMPK (A), mTOR/p-MTOR (B), Akt/p-Akt (C), and JNK/p-JNK (D) were used to detect the respective proteins. Beta actin was used as a loading control. Histograms represent the relative ratios of the phosphorylated proteins to total proteins normalized with the loading control. The figures are representative of at least three individual repetitive experiments. Asterisks indicate significant differences fixed at p ≤ 0.05 (* indicates significant difference relative to control untreated cells, whereas # indicates significant difference relative to LPS-treated cells).