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. 2021 Dec 2;9(12):1814. doi: 10.3390/biomedicines9121814

Figure 3.

Figure 3

Steviol arrests G1 phase of cell-cycle distribution in pancreatic cancer cells. (A,B) Pancreatic cancer cells were seeded and treated with steviol at different time intervals (0, 12, and 24 h). Cell cycle experiment was performed to determine the effect of steviol (10 mM) on G1 cell cycle arrest in HPAF-II (Ai,Bi) and AsPC1 cells (Aii,Bii); (C) steviol modulates the key proteins involved in G1 phase of cell cycle arrest. Pancreatic cancer cells were treated with steviol (10 mM) at different time points (0, 3, 6, and 9 h). Whole-cell lysates were prepared and subjected to Western blotting. The expression level of important cell cycle proteins (p 18, p 21, and Cyclin D1) were evaluated in HPAF-II (Ci) and AsPC1 cells (Cii); the numbers over the blots represent the relative quantification of band intensities with the internal control (β-actin).