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. 2021 Nov 24;9(12):1758. doi: 10.3390/biomedicines9121758

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of VitD3-tolDC in presence/absence of IFN-beta on the differentiation of Th2 and Th17 cells. Percentage of Th2 (CD3+ CD4+ CXCR3- CCR6-) lymphocytes (green) and Th17 (CD3+ CD4+ CXCR3- CCR6+) lymphocytes (gray) from (A) relapsing-remitting multiple sclerosis (RRMS) patients (n = 6) and (B) healthy donors (HD) (n = 6). The percentages of Th2 and Th17 lymphocyte subpopulations were analyzed on PBMC co-cultured with mature DC (mDC), 100%VitD3-tolDC or 50%VitD3-tolDC, in presence/absence of IFN-beta after 5 days of culture. Data represent the relative percentage of each lymphocyte subpopulation in relation to total CD4+ T cells. Error bars correspond to SEM; * p < 0.05.