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. 2021 Nov 23;10(12):1863. doi: 10.3390/antiox10121863

Table 2.

Emerging non-thermal technologies, processing conditions, and effects on the extraction of anthocyanin.

Emerging Non-Thermal Technology Processing Mode Product/Conditions Effect Extraction of Anthocyanins Reference
HHP Discontinuous Grape skins
70 °C, 600 MPa
↑extraction
↑antioxidant activity
+23% [23]
Grapes
<30 °C, 200–550 MPa, 10 min
↑extraction
Migration of anthocyanins to pulp and seeds
+80% [24]
Grape by-products
70 °C, 600 MPa
↑extraction
Phenols +50%
↑antioxidant activity ×3
+41% [26]
UHPH Continuous Grape juice
300 MPa, 78 °C, <0.2 s
↑extraction
↑antioxidant activity
+2.6% [47]
PEF Continuous Grapes
3 kV/cm, 50 pulses
↑extraction
↑juice yield +5%
×3 [48]
Discontinuous Grape by-products
Exponential decay pulses, 70 °C, 30 kV/cm, 10 kJ/kg, 30 pulses, 2 Hz, 15 s
↑extraction
Phenols +50%↑antioxidant activity ×4
+77% [26]
Discontinuous Mazuelo grapes
Exponential decay pulses, <30 °C, 2, 5 and 10 kV/cm, 0.4, 1.8, and 6.7 kJ/kg, 50 pulses, 1 Hz
↑extraction
Phenols +20−31%
+20.3, 28.6 and 41.8% after 120 h [49]
Discontinuous Pinot noir grapes
Square wave bipolar pulses, 1.5 kV/cm, 15 and 70 kJ/kg, 50 Hz, pulse width of 20 μs, pulse numbers of 243 and 1033
↑extraction
↑Phenols
↑bioprotective capacity
+43−74% after 2 days [50]
Continuous Cabernet sauvignon grapes
Square pulses, width of 3 μs, collinear chamber, 2, 5 and 7 kV/cm; 0.56, 3.67, 6.76 kJ/kg, <23 °C, 50 pulses, 122 Hz
Flow 118 kg/h. Average residence time 0.41 s
↑extraction
Phenols +14−36%
+18−45% after 24 h [51]
Continuous Garnacha grapes
Square pulses, width of 3 μs, collinear chamber, 4.3 kV/cm, 60 μs
Flow 1900 kg/h. Average residence time 0.41 s
↑extraction
Phenols +23%
+25% after 7 days [52]
Continuous Merlot grapes
>30 kV/cm, 4.7–49.4 kJ/L
Flow 500 kg/h
↑extraction
↑Phenols +23−162%
Shortening of cold macerations
+17−636% after 24 h [53]
Continuous Rondinella grapes
Square-wave pulses at 1.5 kV/cm, 1, 5 and 10 μs, 2, 10 and 20 kJ/kg, 400 Hz
Flow 250 L/h
↑extraction
↑Phenols +37%
+30% color intensity after fermentation [54]
Continuous Grenache grapes
Pulses of 5 kV/cm, 63.4 kJ/kg. 1800 µS (45 pulses of 40 µS) Average residence time 0.38 s. Temperature < 32 ± 2
Flow 120 kg/h
↑extraction
↑Phenols ×1.6
×2.2 after 24 h [55]
Ultrasounds Discontinuous Tannat grape pomace. US bath: 15−60 °C, 0−100 W, 5−50 min ↑extraction
↑Phenols +50%
+50% [56]
Discontinuous Red grape pomace US bath:
25 °C, 160 W, 40 kHz, 30 min, 0.4 W/mL, 720 J/mL
↑extraction +59% after 5 min [33]
Discontinuous Monastrell grapes
US bath: 18 °C, 40 kHz, 280 W, 90 min
↑extraction
↑Phenols +9%
+8% first day of maceration [57]
Discontinuous Wine lees
Sonifier Cell Disruptor Model 450, high gain horn of ¾″ of diameter. Time of sonication 30–90 s
≈extraction
Lower time
33% of the control time [58]
Discontinuous Grape pomace Moldova variety
ultrasonic transducer coupled with a function generator
↑extraction +18% from 12.5 to 25 kHz [59]
β-irradiation Continuous Tempranillo grapes
10-MeV, 50-kW Rhodotron accelerator, scan frequency of 100 Hz
oses of 0 (control), 0.5, 1 and 10 kGy
↑extraction +71% at 10 kGy [37]