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. 2021 Dec 20;10(12):1356. doi: 10.3390/biology10121356

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The effect of SBD-2 expression on the adhesion of E. coli F17 to OIECs. (A) Overexpression efficiency of SBD-2 mRNA expression level detected by RT-PCR in pGH (Control) cells and pGH-SBD-2 cells. (B) Overexpression efficiency of SBD-2 protein expression level detected by ELISA in pGH (Control) cells and pGH-SBD-2 cells. (C) Adhesion of the F17 fimbria to OIECs transfected with pGH-SBD-2 compared with pGH (Control) analyzed by bacteria enumeration. (D) Interference efficiency of SBD-2 mRNA expression level detected by RT-PCR in OIECs transfected with siRNA-SBD-2 compared with negative control. (E) Interference efficiency of SBD-2 protein expression level detected by ELISA in OIECs transfected with siRNA-SBD-2 compared with negative control. (F) Adhesion of the F17 fimbria to OIECs transfected with siRNA-SBD-2 compared with negative control analyzed by bacteria enumeration. ** p < 0.01, extremely significant difference; NS = no difference. Data were shown as mean ± SD, n = 3 biological replicates.