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. 2021 Dec 17;10(12):1345. doi: 10.3390/biology10121345

Figure 2.

Figure 2

The interaction between Atu0526 and CheWs. (A) Bacterial two-hybrid for testing the interactions between Atu0526 and CheWs. The bacterial cells harboring positive control proteins or different combinations of Atu0526 and CheWs were spotted on LB-CTCK plates with X-GAL. Bacterial cells can grow on the plate and appear blue, indicating an interaction between proteins. (B) Pull-down assay for testing the interactions between Atu0526 and CheWs. The 6×His-tagged Atu0526 and SalT were incubated with the crude extraction of A. fabrum C58 and then pulled down by Ni-IDA Resins. The eluted solutions were subsequently immunoblotted with the antibodies against two CheWs [42] Original blot images in Supplementary Figures S1 and S2.