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Structure prediction for circRNAs based on their −∆G/nt. Three different structure categories are defined: undetermined structure, highly structured and poorly structured. (A) Structure distribution of the circRNAs detected in EVs from RR-MS, SP-MS or HC individuals. Significant difference is found between SP-MS patients and HCs (p = 0.0036). (B) Comparison of the structure distribution between EVs and leukocytes, showing a significant difference between them (p < 0.001).
