TABLE 3.
Oligonucleotides used for real-time PCR assay
| Primer, product, or probe (sequence) | Virus and positions amplifieda | Length (nucleotides) | G+C content (%) | Melting temp (°C) |
|---|---|---|---|---|
| Primers | ||||
| Forward (5′-CATCACCGACCCGGAGAGGGAC) | HSV-1, 65866–65887; HSV-2; 66339–66360 | 22 | 68.2 | 66.9 |
| Reverse (5′-GGGCCAGGCGCTTGTTGGTGTA) | HSV-1, 65936–65957; HSV-2, 66409–66430 | 22 | 63.6 | 69.0 |
| Product | HSV-1, 65866–65957; HSV-2, 66430–66339 | 92 | ||
| TaqMan probe (5′-6FAM-CCGCCGAACTGAGCAGACACCCGCGC-TAMRA) | HSV-1, 65907–65932; HSV-2, 66380–66405 | 26 | 73.1 | 79.8 |