Skip to main content
. 2021 Nov 3;322(1):G21–G33. doi: 10.1152/ajpgi.00266.2021

Figure 3.

Figure 3.

Ischemia-reperfusion injury and innate immunity in functional and nonfunctional livers. A: heatmap displays enrichment of canonical pathways related to innate immunity derived from IPA. The significance threshold was set to −log10 P value > 7 for comparisons of 3- and 6-h gene sets to preperfusion (0 h) baseline for each group. Lobular (B) and portal (C) inflammatory infiltrate as scored by a blinded pathologist. Representative H&E images for functional and nonfunctional livers are displayed. Scale bars: 200 µm (lobular), 100 µm (portal). D: damage-associated molecular pattern (HMGB1) and oxidative stress (8-OHdG) levels in circulating perfusate. Liver F2 6-h perfusate sample not available. E: perfusate levels of cytokines involved in IRI response. Liver F2 6-h perfusate sample not available. F, functional livers (green); H&E; hematoxylin-eosin; HMGB1, high mobility group box 1; IFNγ, interferon γ; IL-6, interleukin-6; IPA, ingenuity pathway analysis; JAK/STAT, Janus kinase/signal transducer and activator of transcription; N, nonfunctional livers (red); NF-κB, nuclear factor-κβ subunit; 8-OHdG, 8-hydroxy-2′deoxyguanosine; TNFα, tumor necrosis factor α.