Figure 3.

(A) The constraint-based multiple alignment (COBALT) for the targets that showed the greatest affinity for dexamethasone. (B) A unique region containing highly conserved residues (red) and moderately conserved residues (blue) were identified. These regions were conserved across NF-κB, INFAR, IL-6R, INF-γ, IFNGR, IL-21R, IL-21, IL-12, TGFβ-1, and CCL5. This fingerprint region (red, blue, grey) appeared to be involved in the high affinity biding of dexamethasone, as seen in the 3D ligand interaction diagrams: (C) IL-6R; (D) IFNAR; (E) IL-21R; (F) INFG.