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Subcellular localization of LEA7, LEA25, LEA42, and LEA43 in (A) ER, (B) nucleus, (C) plastids, and (D) mitochondria in embryos of dry seeds. proLEA_4::LEA_4:Venus lines were either crossed with an organelle marker line with CFP fluorescence (ER-ck or Pt-ck) or the dissected embryos were stained using organelle-specific dyes (DAPI or Mitotracker). Bars indicate 10 µm. Additional LEA proteins with similar localization are shown in Supplementary Figures S1–S3.
