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. 2021 Dec 16;10(12):1335. doi: 10.3390/biology10121335

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Western blot and densitometric analysis of Filamin A after siRNA mediated knockdown of filamin A. (A) Western blot of Filamin A and actin after mDCT15 cells were transiently transfected with filamin A siRNA or control siRNA and then harvested for protein. (B) Densitometric analysis of the immunoreactive filamin A band normalized to actin from panel A. (C) Immunocytochemistry image of mDCT15 cells probed for filamin A flurescence using a rabbit polyclonal filamin A primary antibody and an antirabbit 488 conjugated secondry antibody. (D) Densitometric analysis of the images in panel (C). Three independent experiments were performed, N = 3. * represents p < 0.05.