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. 2021 Dec 16;10(12):1335. doi: 10.3390/biology10121335

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of the CaMKII inhibitor KN93 on total NCC (TNCC) protein expression. (A) Western blot from the samples of three independent experiments (N = 3) after mDCT15 cells were treated with the vehicle (MOCK) or the CaMKII inhibitor KN93 for 4 h before harvesting the cells for protein and isolating the membrane fraction. (B) Summary bar graph of the densitometric analysis for the Western blot in panel (A). The band intensities were quantified after normalizing the immunoreactive NCC bands to actin. * represents p < 0.05.