Table 1.
Primers and probes used in the current study.
| Locus | Primer, Probes | Accession Number | 5′→3′ Sequence | Size |
|---|---|---|---|---|
| mtDNA | NC_005089.1 | Primers for LA-QPCR | ||
| for | GCCAGCCTGACCCATAGCCATAATAT | |||
| rev | GAGAGATTTTATGGGTGTAATGCGG | 10.9 kb | ||
| nDNA | for |
NC_000073.7 X14061.1 |
TTGAGACTGTGATTGGCAATGCCT | |
| rev | CCTTTAATGCCCATCCCGGACT | 8.7 kb | ||
| mtDNA | for | NC_005089.1 | CCCAGCTACTACCATCATTCAAGT | |
| rev | GATGGTTTGGGAGATTGGTTGATGT | 117 bp | ||
| nDNA | for |
NC_000071.7 NM_007393.5 |
CTGCCTGACGGCCAGG | |
| rev | GGAAAAGAGCCTCAGGGCAT | 110 bp | ||
| ND4 | NC_005089.1 | Primers for quantitative analysis of mtDNA/nDNA | ||
| for | ATTATTATTACCCGATGAGGGAACC | |||
| rev | ATTAAGATGAGGGCAATTAGCAGT | |||
| probe | FAM-ACGCCTAAACGCAGGGATTTATTTCCTA-BHQ1 | 115 bp | ||
| GAPDH | for |
NC_000072.7 NM_001289726.1 |
GTGAGGGAGATGCTCAGTGT | |
| rev | CTGGCATTGCTCTCAATGAC | |||
| probe | ROX-TAAGAAACCCTGGACCACCCACCCC-BHQ2 | 214 bp | ||
| ND3 | NC_005089.1 | Primers for mtDNA mutant copies | ||
| for | AGCTCTCCATTTATTGATGAGG | |||
| rev | GAGGTTGAAGAAGGTAGATGGC | 534 bp |