Table 1.

Comparison between different types of lung cancer study models.

	Lung cancer cell lines	PDX model	Organoids model
Advantage	Easy handling; low cost; rapid culturing; large-scale usage.	Well retain genomic and phenotype of original patient specimens.	Well maintain the biology and genetic characteristics of original cancer; well mimic the microenvironment of initial tumor; less time consuming than PDX model; could be genetically modify.
Disadvantage.	Heterogeneous between different lab; genetic drift and clonal selection after long-term layer culture; lost the key characteristic of original tumor.	Expensive and time-consuming; limited passage number; lack of human TME cells.	Lack of standardized methodology; lack of vasculature component; more difficult of long-term initial and propagation.