Figure 2.

Analyses of the TIL clones. (A) ELISA was performed on the supernatant of the clones following stimulation with anti-CD3 and anti-CD28 antibodies for 48 h. The indicated cytokines were analyzed. Results are reported as mean ± standard error of mean (SEM) of duplicate samples from three experiments. (B–D) Real-time RT-PCR of the clones. Basal expression of IL-22, IL-8, GATA3, and RORC is reported as mean of 2^−ΔCt × 10⁵ values of three independent experiments ± SEM.