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. 2021 Dec 20;10(12):2020. doi: 10.3390/antiox10122020

Figure 3.

Figure 3

Near total deletion of CCN2 resulted in reduced p53 activation 3 days after IRI. (A) Representative micrographs of mouse renal cortex stained with phosphorylated p53 (p-p53). Quantification of p-p53 staining showed that increased p53 activation in IRI kidneys, decreased in CCN2 KO mice compared with WT mice. (B) qPCR analysis showed that increased mRNA expression of GADD45A in IRI kidneys reduced in CCN2 KO mice compared with WT mice. Data are expressed as the mean ± SEM (n = 4–5 for WT sham; n = 7 for WT IRI; n = 4–5 for KO sham; n = 10 for KO IRI). TBP was used as an internal control. * p < 0.05 and *** p < 0.001. Bar = 100 µm.